Join the next SynBio workshop -
Wednesday 18th of June

Subject: Introduction to CRISPR/Cas9 gene editing.

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(spots are limited)

Agenda

  • 17:15 - 17:25 Introduction, risks and safety

  • 17:25 - 18:25 Transforming, plating and recovery incubation.

  • 18:25 - 18:45 Break

  • 18:45 - 19:40 Explanation and observations.

  • 19:40 - 20:00 Cleaning up and ethics discussion.

This workshop involves bacterial transformation using the CRISPR/Cas9 system to knock out the lacZ gene in E. coli (DH5α strain) using the miniPCR Knockout! lab kit. The activity includes handling plasmids, culturing genetically modified organisms, and interpreting phenotypic changes via colorimetric screening (X-gal).